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mouse breast carcinoma 4t1 cells  (ATCC)


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    ATCC mouse breast carcinoma 4t1 cells
    Mouse Breast Carcinoma 4t1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 6821 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+breast+carcinoma+4t1+cells/4T1/pmc13179769-74-11-16
    Average 99 stars, based on 6821 article reviews
    mouse breast carcinoma 4t1 cells - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Multiple Displacement Amplification:

    Article Title: Trifluridine selectively inhibits cell growth and induces cell apoptosis of triple-negative breast cancer
    Article Snippet: .. Cells Human mammary epithelial MCF-10A, luminal A subtype of breast cancer cells MCF-7, TNBC subtype of breast cancer cells MDA-MB-231, BT-549 and Hs578T and mouse breast carcinoma 4T1 cells were obtained from American Type Culture Collection (ATCC, VA, USA). .. MCF-10A were cultured in DMEM/F12 medium (Hyclone) supplemented with 5% horse serum (Hyclone), 1% penicillin/streptomycin (Hyclone), 0.5% μg/ml hydrocortisone (Sigma, H-0888), 10 μg/ml insulin (Sigma, I-1882) and 20 ng/ml recombinant human EGF (Peprotech, 100-15).

    Article Title: Trifluridine selectively inhibits cell growth and induces cell apoptosis of triple-negative breast cancer
    Article Snippet: .. Human mammary epithelial MCF-10A, luminal A subtype of breast cancer cells MCF-7, TNBC subtype of breast cancer cells MDA-MB-231, BT-549 and Hs578T and mouse breast carcinoma 4T1 cells were obtained from American Type Culture Collection (ATCC, VA, USA). .. MCF-10A were cultured in DMEM/F12 medium (Hyclone) supplemented with 5% horse serum (Hyclone), 1% penicillin/streptomycin (Hyclone), 0.5% μg/ml hydrocortisone (Sigma, H-0888), 10 μg/ml insulin (Sigma, I-1882) and 20 ng/ml recombinant human EGF (Peprotech, 100-15).

    Cell Culture:

    Article Title: GBVAM as a Novel NHE1 Inhibitor Alleviates Doxorubicin-Induced Cardiotoxicity via PI3K/Akt/mTOR Pathway
    Article Snippet: .. The rat cardiomyocytes H9c2 cells, human breast carcinoma MCF-7 cells and mouse breast carcinoma 4T1 cells (ATCC, America) were cultured with DMEM or RPMI 1640 (C11995500BT or C11875500BT, Gibco) added with 10% FBS (FSP500, ExCell bio) and 1% penicillin-streptomycin ( PB180120 , Solarbio). ..

    Article Title: Multifunctional theragnostic ultrasmall gold nanodot-encapsuled perfluorocarbon nanodroplets for laser-focused ultrasound sequence irradiation (LFSI)-based enhanced tumor ablation.
    Article Snippet: Despite the substantial potential of focused ultrasound therapy, its efficacy in cancer therapy has been limited due to the high cavitation threshold and safety concerns regarding the use of high-intensity energy pulses.. Here, ultrasmall Au nanodot-loaded PEG-modified perfluorocarbon nanodroplets (Au-PFCnDs) were prepared and used as a therapeutic enhancer.. A LFSI method was designed to achieve enhanced tumor ablation at a mild focused ultrasound (FUS) energy pulse with the assistance of the instinct photothermal effect of intratumor-permeable ultrasmall Au nanodots under 808 nm NIR laser irradiation.

    Expressing:

    Article Title: Lysyl oxidase-like 2 (LOXL2), a new regulator of cell polarity required for metastatic dissemination of basal-like breast carcinomas
    Article Snippet: .. Firstly, we reassessed the expression of LOXL2 and other LOX members in the mouse breast carcinoma 4T1 cells that were not included in our original ms. We have obtained 4T1 cells from the ATCC and analysed the expression of the five LOX family members by RT-PCR and qPCR. ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Lysyl oxidase-like 2 (LOXL2), a new regulator of cell polarity required for metastatic dissemination of basal-like breast carcinomas
    Article Snippet: .. Firstly, we reassessed the expression of LOXL2 and other LOX members in the mouse breast carcinoma 4T1 cells that were not included in our original ms. We have obtained 4T1 cells from the ATCC and analysed the expression of the five LOX family members by RT-PCR and qPCR. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Lysyl oxidase-like 2 (LOXL2), a new regulator of cell polarity required for metastatic dissemination of basal-like breast carcinomas
    Article Snippet: .. Firstly, we reassessed the expression of LOXL2 and other LOX members in the mouse breast carcinoma 4T1 cells that were not included in our original ms. We have obtained 4T1 cells from the ATCC and analysed the expression of the five LOX family members by RT-PCR and qPCR. ..



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    FIGURE 3 | AEP enhances the level of epithelial mesenchymal transition in <t>breast</t> <t>cancer</t> cells by regulating CD74. (A) Expression of related pathway protein after administration of BIC-113 to MCF-7 cells; (B) Protein expression of related pathways after administration of BIC-113 to MDA- MB-231 cells; (C) Protein expression of related pathways in MCF-7 AEP OE <t>cell</t> <t>line</t> and MCF-7 WT cell line; (D) Protein expression of related path- ways after administration of siCD74 to MCF-7 WT cells and MCF-7 shAEP cells.
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    FIGURE 3 | AEP enhances the level of epithelial mesenchymal transition in breast cancer cells by regulating CD74. (A) Expression of related pathway protein after administration of BIC-113 to MCF-7 cells; (B) Protein expression of related pathways after administration of BIC-113 to MDA- MB-231 cells; (C) Protein expression of related pathways in MCF-7 AEP OE cell line and MCF-7 WT cell line; (D) Protein expression of related path- ways after administration of siCD74 to MCF-7 WT cells and MCF-7 shAEP cells.

    Journal: Journal of cellular and molecular medicine

    Article Title: The Combination of MIF Inhibitor and AEP Targeted Inhibitor to Reduce Lung Metastasis in Breast Cancer and Its Mechanism.

    doi: 10.1111/jcmm.70616

    Figure Lengend Snippet: FIGURE 3 | AEP enhances the level of epithelial mesenchymal transition in breast cancer cells by regulating CD74. (A) Expression of related pathway protein after administration of BIC-113 to MCF-7 cells; (B) Protein expression of related pathways after administration of BIC-113 to MDA- MB-231 cells; (C) Protein expression of related pathways in MCF-7 AEP OE cell line and MCF-7 WT cell line; (D) Protein expression of related path- ways after administration of siCD74 to MCF-7 WT cells and MCF-7 shAEP cells.

    Article Snippet: HEK293T (RRID:CVCL_0063), mice breast cancer cell line (4 T1.2 (ATCC; CRL- 3406), 4 T1 (ATCC; CRL- 2539)) and human breast cancer cell line (MDA- MB- 231 (ATCC; CRM- HTB- 26), MCF- 7 (ATCC; HTB- 22)) were obtained from the American Type Culture Collection (ATCC, Manassas, VA).

    Techniques: Expressing

    FIGURE 2 | AEP enhances the migration ability of breast cancer cells by regulating CD74. (A) Scratching experiments on different groups of 4 T1.2 cells after administration of siCD74; scale bar is 100 μm; (B) Statistics of the results of scratching experiments on different groups of 4 T1.2 cells; (C) Transwell migration experiments on different groups of 4 T1.2 cells after administration of siCD74; scale bar is 100 μm; (D) Statistics of the results of transwell migration experiments on different groups of 4 T1.2 cells. Compared with the control group, *p < 0.05, **p < 0.01.

    Journal: Journal of cellular and molecular medicine

    Article Title: The Combination of MIF Inhibitor and AEP Targeted Inhibitor to Reduce Lung Metastasis in Breast Cancer and Its Mechanism.

    doi: 10.1111/jcmm.70616

    Figure Lengend Snippet: FIGURE 2 | AEP enhances the migration ability of breast cancer cells by regulating CD74. (A) Scratching experiments on different groups of 4 T1.2 cells after administration of siCD74; scale bar is 100 μm; (B) Statistics of the results of scratching experiments on different groups of 4 T1.2 cells; (C) Transwell migration experiments on different groups of 4 T1.2 cells after administration of siCD74; scale bar is 100 μm; (D) Statistics of the results of transwell migration experiments on different groups of 4 T1.2 cells. Compared with the control group, *p < 0.05, **p < 0.01.

    Article Snippet: HEK293T (RRID:CVCL_0063), mice breast cancer cell line (4 T1.2 (ATCC; CRL- 3406), 4 T1 (ATCC; CRL- 2539)) and human breast cancer cell line (MDA- MB- 231 (ATCC; CRM- HTB- 26), MCF- 7 (ATCC; HTB- 22)) were obtained from the American Type Culture Collection (ATCC, Manassas, VA).

    Techniques: Migration, Control

    FIGURE 4 | Exogenous AEP enhances the level of epithelial mesen- chymal transition in breast cancer cells. (A) Cell co-culture model; (B) Protein expression of 293 T WT and 293 T shAEP cells; (C) AEP levels in the supernatant of 293 T WT and 293 T shAEP cells; (D) Expression of MCF-7 cell-associated proteins after co-culture with different groups of 293 T cells. Compared with the control group. *p < 0.05.

    Journal: Journal of cellular and molecular medicine

    Article Title: The Combination of MIF Inhibitor and AEP Targeted Inhibitor to Reduce Lung Metastasis in Breast Cancer and Its Mechanism.

    doi: 10.1111/jcmm.70616

    Figure Lengend Snippet: FIGURE 4 | Exogenous AEP enhances the level of epithelial mesen- chymal transition in breast cancer cells. (A) Cell co-culture model; (B) Protein expression of 293 T WT and 293 T shAEP cells; (C) AEP levels in the supernatant of 293 T WT and 293 T shAEP cells; (D) Expression of MCF-7 cell-associated proteins after co-culture with different groups of 293 T cells. Compared with the control group. *p < 0.05.

    Article Snippet: HEK293T (RRID:CVCL_0063), mice breast cancer cell line (4 T1.2 (ATCC; CRL- 3406), 4 T1 (ATCC; CRL- 2539)) and human breast cancer cell line (MDA- MB- 231 (ATCC; CRM- HTB- 26), MCF- 7 (ATCC; HTB- 22)) were obtained from the American Type Culture Collection (ATCC, Manassas, VA).

    Techniques: Co-Culture Assay, Expressing, Control

    FIGURE 5 | Combined inhibition of AEP and CD74 can reduce the migration ability of breast cancer cells and the level of epithelial mesenchymal transformation. (A) Cell viability of MCF-7 cell line with different dosing regimens; (B) Results of wound healing assay of MCF-7 cell line, scale bar is 100 μM; (C) Results of transwell migration assay of MCF-7 cell line, scale bar is 100 μM; (D, E) Statistics of wound healing assay or transwell migra- tion assay of MCF-7 cell line; Compared with the control group. *p < 0.05, **p < 0.01, ns p > 0.05; (F) Combined inhibition of AEP and CD74 reduces the level of epithelial mesenchymal transition in breast cancer cells.

    Journal: Journal of cellular and molecular medicine

    Article Title: The Combination of MIF Inhibitor and AEP Targeted Inhibitor to Reduce Lung Metastasis in Breast Cancer and Its Mechanism.

    doi: 10.1111/jcmm.70616

    Figure Lengend Snippet: FIGURE 5 | Combined inhibition of AEP and CD74 can reduce the migration ability of breast cancer cells and the level of epithelial mesenchymal transformation. (A) Cell viability of MCF-7 cell line with different dosing regimens; (B) Results of wound healing assay of MCF-7 cell line, scale bar is 100 μM; (C) Results of transwell migration assay of MCF-7 cell line, scale bar is 100 μM; (D, E) Statistics of wound healing assay or transwell migra- tion assay of MCF-7 cell line; Compared with the control group. *p < 0.05, **p < 0.01, ns p > 0.05; (F) Combined inhibition of AEP and CD74 reduces the level of epithelial mesenchymal transition in breast cancer cells.

    Article Snippet: HEK293T (RRID:CVCL_0063), mice breast cancer cell line (4 T1.2 (ATCC; CRL- 3406), 4 T1 (ATCC; CRL- 2539)) and human breast cancer cell line (MDA- MB- 231 (ATCC; CRM- HTB- 26), MCF- 7 (ATCC; HTB- 22)) were obtained from the American Type Culture Collection (ATCC, Manassas, VA).

    Techniques: Inhibition, Migration, Transformation Assay, Wound Healing Assay, Transwell Migration Assay, Control

    FIGURE 6 | Combined inhibition of AEP and CD74 to overcome breast cancer lung metastasis. (A) Experimental protocol for breast cancer lung metastasis animals; (B) Body weight statistics of mice in each group; (C) Photographs of lung metastases in each group of mice, with red arrows indicating lung metastases; (D) The number of lung metastases in each group of mice; (E) HE staining of lung tissues in each group of mice; (F), Immunohistochemistry of AEP, CD74 and E-cadherin in lung tissues of mice in each group. Compared with the control group: *p < 0.05, **p < 0.01.

    Journal: Journal of cellular and molecular medicine

    Article Title: The Combination of MIF Inhibitor and AEP Targeted Inhibitor to Reduce Lung Metastasis in Breast Cancer and Its Mechanism.

    doi: 10.1111/jcmm.70616

    Figure Lengend Snippet: FIGURE 6 | Combined inhibition of AEP and CD74 to overcome breast cancer lung metastasis. (A) Experimental protocol for breast cancer lung metastasis animals; (B) Body weight statistics of mice in each group; (C) Photographs of lung metastases in each group of mice, with red arrows indicating lung metastases; (D) The number of lung metastases in each group of mice; (E) HE staining of lung tissues in each group of mice; (F), Immunohistochemistry of AEP, CD74 and E-cadherin in lung tissues of mice in each group. Compared with the control group: *p < 0.05, **p < 0.01.

    Article Snippet: HEK293T (RRID:CVCL_0063), mice breast cancer cell line (4 T1.2 (ATCC; CRL- 3406), 4 T1 (ATCC; CRL- 2539)) and human breast cancer cell line (MDA- MB- 231 (ATCC; CRM- HTB- 26), MCF- 7 (ATCC; HTB- 22)) were obtained from the American Type Culture Collection (ATCC, Manassas, VA).

    Techniques: Inhibition, Staining, Immunohistochemistry, Control